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Compaction of RNA Hairpins and Their Kissing Complexes in Native Electrospray Mass Spectrometry

preprint
revised on 17.08.2020 and posted on 17.08.2020 by Joséphine Abi-Ghanem, Clémence Rabin, Massimiliano Porrini, Frédéric Rosu, Valerie Gabelica
When electrosprayed from typical native MS solution conditions, RNA hairpins and kissing complexes acquire charge states at which they get significantly more compact in the gas phase than their initial structure in solution. Here we show the limits of using force field molecular dynamics to interpret the gas-phase structures of nucleic acid complexes in the gas phase, and we suggest that higher-level calculation levels should be used in the future.

Funding

ERC-2013-CoG-616551-DNAFOLDIMS

History

Email Address of Submitting Author

v.gabelica@iecb.u-bordeaux.fr

Institution

INSERM

Country

France

ORCID For Submitting Author

0000-0001-9496-0165

Declaration of Conflict of Interest

There is no conflict of interest to declare

Version Notes

August 17, 2020: third revisions

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