Compaction of RNA Hairpins and Their Kissing Complexes in Native Electrospray Mass Spectrometry

03 July 2020, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

When electrosprayed from native solution conditions, RNA hairpins and kissing complexes acquire charge states at which they get significantly more compact in the gas phase than their initial structure in solution. Here we show the limits of using force field molecular dynamics to interpret the gas-phase structures of nucleic acid complexes in the gas phase, and we suggest that higher-level calculation levels should be used in the future.

Keywords

RNA
Kissing Complex
Hairpin
Ion mobility spectrometry
Collision Cross Sections
gas phase ion structures
rearrangement
Native mass spectrometry
electrospray

Supplementary materials

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200703 KC-in-gas revised2 SI
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