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Coding Synthetic Chemistry Strategies into Bacterial Designer Cells

preprint
submitted on 10.05.2021, 11:58 and posted on 11.05.2021, 10:49 by Yu-Chang Liu, Zhong-Liu Wu, Jan Deska

Following a synthetic chemistry blueprint for the valorization of lignocellulosic platform chemicals, this study showcases a so far unprecedented approach to implement non-natural enzyme modules in vivo. For the design of a novel functional whole cell tool, two purely abiotic transformations were incorporated into a recombinant bacterial host that allows production of complex lactone building blocks. This whole cell system streamlines the synthetic cascade, eliminates isolation and purification steps, and provides a high degree stereoselectivity that has so far been elusive in the chemical methodology.

Funding

Beyond Biosynthesis: Enzymes as Catalysts in Non-natural Synthetic Transformations

Academy of Finland

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CEVALOR – Cellular factories for the valorization of biorefinery chemicals

Academy of Finland

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Biosynthetic production of complex lactone building blocks utilizing non-natural enzymatic modules

Novo Nordisk Foundation

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History

Email Address of Submitting Author

jan.deska@aalto.fi

Institution

Aalto University, Department of Chemistry

Country

Finland

ORCID For Submitting Author

0000-0003-4622-2365

Declaration of Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.