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Clickable Cellulosic Surfaces for Peptide-Based Bioassays

revised on 16.05.2019, 10:40 and posted on 16.05.2019, 14:16 by Maria Teresa Odinolfi, Alessandro Romanato, Greta Bergamaschi, Alessandro Strada, Laura Sola, Alessandro Girella, Chiara Milanese, Marcella Chiari, Alessandro Gori, Marina Cretich
The use of peptides in paper-based analytics is a highly appealing field, yet it suffers from severe limitations. This is mostly due to the loss of effective target recognition properties of this relatively small bioprobes upon nonspecific adsorption onto cellulose substrates. Here, we address this issue by introducing a simple polymer-based strategy to obtain clickable cellulosic surfaces, that we exploited for the chemoselective bioconjugation of peptide bioprobes. Our method largely outperformed standard adsorption-based immobilization strategy in a challenging, real-case immunoassay, namely the diagnostic discrimination of Zika+ individuals from healthy controls. Of note, the clickable polymeric coating not only allows efficient peptides bioconjugation, but it provides favorable anti-fouling properties to the cellulosic support. We envisage our strategy to broaden the repertoire of cellulosic materials manipulation and promote a renewed interest in peptide-based paper bioassays.


Work partially funded by Regione Lombardia, project READY (Regional Network for developing diagnostic methods in rapid response to emerging epidemics and bio-emergencies) ID 229472.

PRIN 2015JTL4HL is gratefully acknowledged for financial support to A. S.


Email Address of Submitting Author


Consiglio Nazionale delle Ricerche, Istituto di Chimica del Riconoscimento Molecolare (ICRM)



ORCID For Submitting Author


Declaration of Conflict of Interest

No conflict of interest