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YOYO1_TA_ChenJ_20170923.pdf (1.19 MB)
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Charge Transfer Initializes Photoexcited YOYO-1 Intramolecular Rotation and Fluorescent Quenching

preprint
submitted on 23.10.2017 and posted on 25.10.2017 by Jixin Chen, Lei Wang, Joseph R. Pyle
YOYO-1 is a commonly used cyanine dye for DNA staining that is fluorescently bright in DNA but very dim in water. The major assumption of its excited electron decay pathway is thermal relaxation via the rotation at a bridging methine that connects the two moieties of the molecule, i.e. photo-isomerization. In this report, we use femtosecond transient absorption spectroscopy to directly measure the excited electron decay, the hole refill, and the hot ground state rise and decay. The data suggest that the first step of the photo-isomerization involves a charge transfer to quench the holes and vibrational activation of the molecules to a hot ground state.

History

Topic

  • Photochemistry (Physical Chem.)
  • Spectroscopy (Physical Chem.)

Email Address of Submitting Author

chenj@ohio.edu

Institution

Ohio University

Country

USA

ORCID For Submitting Author

0000-0001-7381-0918

Declaration of Conflict of Interest

None

Exports