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Cellular Uptake and Magneto-Hyperthermia Induced Cytotoxicity using Photoluminescent Fe3O4 Nanoparticle/Si Quantum Dot Hybrids

preprint
submitted on 23.10.2020, 19:44 and posted on 27.10.2020, 09:34 by Morteza Javadi, Van A. Ortega, Alyxandra Thiessen, Maryam Aghajamali, Muhammad Amirul Islam, Jenna M. Drummond, Greg G. Goss, Jonathan G.C. Veinot

The design and fabrication of Si-based multi-functional nanomaterials for biological and biomedical applications is an active area of research. The potential benefits of using Si-based nanomaterials are not only due to their size/surface-dependent optical responses but also the high biocompatibility and low-toxicity of silicon itself. Combining these characteristics with the magnetic properties of Fe3O4 nanoparticles (NPs) multiplies the options available for real-world applications. In the current study, biocompatible magnetofluorescent nano-hybrids have been prepared by covalent linking of Si quantum dots to water-dispersible Fe3O4 NPs via dicyclohexylcarbodiimide (DCC) coupling. We explore some of the properties of these magnetofluorescent nano-hybrids as well as evaluate uptake, the potential for cellular toxicity, and the induction of acute cellular oxidative stress in a mast cells-like cell line (RBL-2H3) by heat induction through short-term radio frequency modulation (10 min @ 156 kHz, 500 A). We found that the NPs were internalized readily by the cells and also penetrated the nuclear membrane. Radio frequency activated nano-hybrids also had significantly increased cell death where > 50% of the RBL-2H3 cells were found to be in an apoptotic or necrotic state, and that this was attributable to increased triggering of oxidative cell stress mechanisms.

History

Email Address of Submitting Author

aarbo@ualberta.ca

Institution

University of Alberta

Country

Canada

ORCID For Submitting Author

0000-0001-9978-1422

Declaration of Conflict of Interest

No conflict of interest

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