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Cationic Biphotonic Lanthanide Luminescent Bioprobes Based on Functionalized Cross-Bridged Cyclam Macrocycles

preprint
submitted on 13.01.2020 and posted on 22.01.2020 by Jonathan Mendy, Amandine Roux, Jean-Christophe Mulatier, Alain Duperray, Alexei Grichine, Yannick Guyot, Sophie Brasselet, François Riobé, Chantal Andraud, Boris Le Guennic, Véronique Patinec, Raphael Tripier, Maryline Beyler, Olivier MAURY, Anh Thy Bui, Damien Curton

Cationic lanthanide complexes are generally able to spontaneously internalize into living cells. Following our previous works based on diMe-cyclen framework, a second generation of cationic water-soluble lanthanide complexes based on a constrained cross-bridged cyclam macrocycle functionalized with donor-p-conjugated picolinate antennas has been prepared with europium(III) and ytterbium(III). Their spectroscopic properties were thoroughly investigated in various solvents and rationalized with the help of DFT calculations. A significant improvement is observed in the case of the Eu3+ complex, while the Yb3+ analogous conserve an excellent brightness in aqueous solvent. Two-photon (2P) microscopy imaging experiments on living T24 human cancer cells confirmed the spontaneous internalization of the probes and images with good signal-to-noise have been obtained in the classical NIR-to-visible configuration with Eu3+ luminescent bioprobe and in the NIR-to-NIR with the Yb3+ one.

History

Email Address of Submitting Author

olivier.maury@ens-lyon.fr

Institution

Ecole Normale supérieure de Lyon

Country

France

ORCID For Submitting Author

0000-0002-4639-643X

Declaration of Conflict of Interest

no conflict of interest

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