ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
1/1
3 files

Branched Kissing Loops for the Construction of Diverse RNA Homooligomeric Nanostructures

preprint
submitted on 13.03.2019, 15:19 and posted on 14.03.2019, 15:42 by Di Liu, Cody Geary, Gang Chen, Yaming Shao, Mo Li, Chengde Mao, Ebbe S. Andersen, Joseph A. Piccirilli, Paul W. K. Rothemund, Yossi Weizmann
This study presents a robust homooligomeric self-assembly system based on RNA and DNA tiles that are folded from a single-strand of nucleic acid and assemble via a novel artificially designed branched kissing-loops (bKL) motif. By adjusting the geometry of individual tiles, we have constructed a total of 16 different structures that demonstrate our control over the curvature, torsion, and number of helices of assembled structures. Furthermore, bKL-based tiles can be assembled cotranscriptionally, and can be expressed in living bacterial cells.

Funding

CAREER: Programmable Artificial DNA Topology for Biological and Medical Applications

Directorate for Mathematical & Physical Sciences

Find out more...

Chaperone-Assisted RNA Crystallography

National Institute of General Medical Sciences

Find out more...

ERC grant (683305)

Collaborative Research: Molecular Programming Architectures, Abstractions, Algorithms, and Applications

Directorate for Computer & Information Science & Engineering

Find out more...

Asymmetric DNA Shapes for Orienting Molecular Components within Hybrid Nanodevices

Directorate for Engineering

Find out more...

ONR grant (N00014-16-1-2159)

History

Email Address of Submitting Author

yweizmann@uchicago.edu

Institution

University of Chicago

Country

USA

ORCID For Submitting Author

0000-0002-3467-990X

Declaration of Conflict of Interest

No conflict of interest

Exports