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Bovine Serum Albumin as A Superior “FP-Tag” for High-Throughput Glycosyltransferase Assay

preprint
submitted on 21.08.2020 and posted on 24.08.2020 by Xinjian Yin, Jiaxin Li, Senhua Chen, Yuping Wu, Zhigang She, Lan Liu, Yue Wang, Zhizeng Gao
Here, we report the development of a high-throughput “FP-Tag” OGT assay with bovine serum albumin as a low-cost and superior “FP-Tag”. With this assay, 2-methyleurotinone was identified as a low M-range OGT inhibitor. This type of assay with BSA as “FP-Tag” would find more applications with other glycosyltransferases.

History

Email Address of Submitting Author

gaozhizeng@mail.sysu.edu.cn

Institution

Sun Yat-sen University

Country

China

ORCID For Submitting Author

0000-0002-8176-0727

Declaration of Conflict of Interest

No conflict of interest

Version Notes

first version

Exports