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Bovine Serum Albumin as A Superior “FP-Tag” for High-Throughput Glycosyltransferase Assay

preprint
submitted on 21.08.2020, 12:57 and posted on 24.08.2020, 04:38 by Xinjian Yin, Jiaxin Li, Senhua Chen, Yuping Wu, Zhigang She, Lan Liu, Yue Wang, Zhizeng Gao
Here, we report the development of a high-throughput “FP-Tag” OGT assay with bovine serum albumin as a low-cost and superior “FP-Tag”. With this assay, 2-methyleurotinone was identified as a low M-range OGT inhibitor. This type of assay with BSA as “FP-Tag” would find more applications with other glycosyltransferases.

History

Email Address of Submitting Author

gaozhizeng@mail.sysu.edu.cn

Institution

Sun Yat-sen University

Country

China

ORCID For Submitting Author

0000-0002-8176-0727

Declaration of Conflict of Interest

No conflict of interest

Version Notes

first version