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Biotin as a Reactive Handle to Selectively Label Proteins and DNA with Small Molecules

preprint
submitted on 06.01.2021, 00:06 and posted on 06.01.2021, 13:15 by Adam Cotton, James Wells, Ian Seiple

Here we report the reaction between biotin and azide-labelled oxaziridine reagents in aqueous conditions at room temperature. This method, which we call biotin redox-activated chemical tagging (BioReACT), achieves efficient and stable labelling of proteins with oxaziridine reagents. We functionally validate the method by generating an antibody-drug conjugate and numerous flow-cytometry reagents. Finally, we conjugate a functional click handle to a biotinylated oligonucleotide. These studies show that the biotin–oxaziridine reaction is a powerful approach for the efficient synthesis of stable protein and DNA bioconjugates.

Funding

David and Lucille Packard Foundation

History

Email Address of Submitting Author

adam.cotton@ucsf.edu

Institution

University of California, San Francisco

Country

United States

ORCID For Submitting Author

0000-0002-6988-1569

Declaration of Conflict of Interest

No conflict of interest

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ChemRxiv

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