These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
2 files

Biocatalytic Conversion of 5-Hydroxymethylfurfural by Galactose Oxidase: Toward Scalable Technology Using Integrated Process and Enzyme Engineering

submitted on 07.09.2020, 16:24 and posted on 08.09.2020, 10:08 by William Birmingham, Asbjørn Toftgaard Pedersen, Mafalda Dias Gomes, Mathias Bøje Madsen, Michael Breuer, John Woodley, Nicholas J. Turner
5-Hydroxymethylfurfural (HMF) has emerged as a crucial bio-based chemical building block in the drive towards developing materials from renewable resources, due to its direct preparation from sugars and its readily diversifiable scaffold. A key obstacle in transitioning to bio-based plastic production lies in meeting the necessary industrial production efficiency, particularly in the cost-effective conversion of HMF to valuable intermediates. To address the challenge of developing scalable technology for oxidizing crude HMF to more valuable chemicals, we have integrated process and enzyme engineering to provide a galactose oxidase (GOase) variant with remarkably high activity toward HMF, improved O2 binding and excellent productivity (>1,000,000 TTN). The process concept presented here for GOase catalysed selective oxidation of HMF to 2,5-diformylfuran offers a productive and efficient platform for further development, thereby laying the groundwork for a biocatalytic route to scalable production of furan-based chemical building blocks from sustainable feedstocks.


Developing a validated technology platform for the application of oxygen dependent enzymes in synthesis and transformation of alcohols

European Commission

Find out more...


Email Address of Submitting Author


University of Manchester


United Kingdom

ORCID For Submitting Author


Declaration of Conflict of Interest

No conflict of interest.