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Assembly of Model Membrane Nanodiscs for Native Mass Spectrometry

preprint
submitted on 12.02.2021, 16:10 and posted on 16.02.2021, 12:06 by Marius Kostelic, Ciara K. Zak, Hiruni Jayasekera, Michael Marty
Native mass spectrometry (MS) with nanodiscs is a promising technique for characterizing membrane protein and peptide interactions in lipid bilayers. However, prior studies have used nanodiscs made of only one or two lipids, which lack the complexity of a natural lipid bilayer. To better model specific biological membranes, we developed model mammalian, bacterial, and mitochondrial nanodiscs with up to four different phospholipids. Careful selection of lipids with similar masses that balance the fluidity and curvature enabled these complex nanodiscs to be assembled and resolved with native MS. We then applied this approach to characterize the specificity and incorporation of LL-37, a human antimicrobial peptide, in single lipid nanodiscs versus model bacterial nanodiscs. Overall, development of these model membrane nanodiscs reveals new insights into the assembly of complex nanodiscs and provides a useful toolkit for studying membrane protein, peptide, and lipid interactions in model biological membranes.

Funding

CHE-1845230

History

Email Address of Submitting Author

mtmarty@arizona.edu

Institution

University of Arizona

Country

USA

ORCID For Submitting Author

0000-0001-8115-1772

Declaration of Conflict of Interest

None

Version Notes

Version 1.0.0

Exports

ChemRxiv

Exports