These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
ChemRxiv.pdf (2.18 MB)

Arylphosphonate-Tethered Porphyrins: Fluorescence Silencing Speaks a Metal Language in Living Enterocyte

submitted on 12.12.2020, 09:50 and posted on 14.12.2020, 13:28 by Claudia Keil, Julia Klein, Franz-Josef Schmitt, Yunus Zorlu, Hajo Haase, Gündoğ Yücesan

We report the application of a highly versatile and

engineerable novel sensor platform to monitor biologically significant

and toxic metal ions in live human Caco-2 enterocytes. The extended

conjugation between the fluorescent porphyrin core and metal ions via

aromatic phenylphosphonic acid tethers generates a unique turn off

and turn on fluorescence and, in addition, shifts in absorption and

emission spectra for zinc, cobalt, cadmium and mercury. The reported

fluorescent probes p-H8TPPA and m-H8TPPA can monitor a wide

range of metal ion concentrations via fluorescence titration and also

via fluorescence decay curves. Cu and Zn-induced turn off

fluorescence can be differentially reversed by the addition of common

chelators. Both p-H8TPPA and m-H8TPPA readily pass the

mammalian cellular membrane due to their amphipathic character as

confirmed by confocal microscopic imaging of living enterocytes.




Email Address of Submitting Author


Technische Universität Berlin



ORCID For Submitting Author


Declaration of Conflict of Interest

No conflict of interest