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Arylphosphonate-Tethered Porphyrins: Fluorescence Silencing Speaks a Metal Language in Living Enterocyte

preprint
submitted on 12.12.2020, 09:50 and posted on 14.12.2020, 13:28 by Claudia Keil, Julia Klein, Franz-Josef Schmitt, Yunus Zorlu, Hajo Haase, Gündoğ Yücesan

We report the application of a highly versatile and

engineerable novel sensor platform to monitor biologically significant

and toxic metal ions in live human Caco-2 enterocytes. The extended

conjugation between the fluorescent porphyrin core and metal ions via

aromatic phenylphosphonic acid tethers generates a unique turn off

and turn on fluorescence and, in addition, shifts in absorption and

emission spectra for zinc, cobalt, cadmium and mercury. The reported

fluorescent probes p-H8TPPA and m-H8TPPA can monitor a wide

range of metal ion concentrations via fluorescence titration and also

via fluorescence decay curves. Cu and Zn-induced turn off

fluorescence can be differentially reversed by the addition of common

chelators. Both p-H8TPPA and m-H8TPPA readily pass the

mammalian cellular membrane due to their amphipathic character as

confirmed by confocal microscopic imaging of living enterocytes.

Funding

DFG

History

Email Address of Submitting Author

yuecesan@tu-berlin.de

Institution

Technische Universität Berlin

Country

Germany

ORCID For Submitting Author

0000-0002-5105-7280

Declaration of Conflict of Interest

No conflict of interest

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