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Applications of Mechanochemistry for the Synthesis of DNA on Ionic Liquid Supports

preprint
submitted on 28.12.2020, 11:24 and posted on 29.12.2020, 10:42 by christopher j ohnston, christopher hardacre, marie migaud

The standard methodology for the synthesis of oligonucleotides is the solid-phase method. Here, we report the first case of mechanochemistry being directly applied to the synthesis of a dinucleotide on a soluble support. While mechanochemistry eliminates the need to solubilise a supported oligomer in solution phase for phosphoramidite coupling, the support still confers the benefit of being able to purify the product by precipitation, or by simple liquid-liquid extractions as needed. Furthermore, this study also explores the use of mechanochemistry in the initial functionalisation of the ionic liquid support with a nucleoside monomer.

History

Email Address of Submitting Author

mmigaud@southalabama.edu

Institution

university of south alabamA

Country

united states of America

ORCID For Submitting Author

0000-0002-9626-2405

Declaration of Conflict of Interest

no conflict of interest

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