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An Improved Protocol for the Synthesis and Purification of Yariv Reagents

preprint
submitted on 21.06.2020 and posted on 24.06.2020 by Amit Basu, Raghuraj Hosing, Michael Saladino, Helene Kuhn, David Caianiello, Robert Lusi
Yariv reagents are widely used tools to study plant proteoglycans known as the Arabinogalactan proteins (AGPs). Despite widespread use of the Yariv reagent in the study of AGPs and several reports of synthetic protocols, there remains a lack of a simple procedure to obtain pure Yariv reagents. We report our optimized protocols to address the purification issues faced upon synthesis of Yariv reagents. Additionally, we report challenges that make characterization difficult such as peak broadening in NMR due to Yariv-water interactions. We also show ways of processing Yariv reagents to overcome the characterization issues caused by peak broadening.

Funding

NSF CHE1607554

History

Email Address of Submitting Author

amit_basu@brown.edu

Institution

Brown University

Country

United States

ORCID For Submitting Author

0000-0001-6312-675X

Declaration of Conflict of Interest

No conflict of interest

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