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Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics

preprint
submitted on 30.04.2020 and posted on 04.05.2020 by Dario Brambilla, Laura Sola, Marcella Chiari
An optimized general protocol for DNA-protein ligation is provided and the conjugates are used to convert DNA arrays into antibody microarrays. Arrays obtained through DDI were used to capture and characterize extracellular vesicles (EVs), an emerging class of biomarkers. The proposed platform was tested against commercially available antibody microarrays, showing good performance combined with ease of fabrication.

Funding

European Union Horizon 2020, Grant 766466: INDEX, Integrated Nanoparticle isolation and Detection system for complete on-chip analysis of Exosomes.

History

Email Address of Submitting Author

dario.brambilla@scitec.cnr.it

Institution

Institute of Chemical Science and Technology, National Research Council of Italy (CNR-SCITEC)

Country

Italy

ORCID For Submitting Author

0000-0003-3490-4481

Declaration of Conflict of Interest

no conflict of interest

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