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A Two-Dimensional Metallacycle Cross-Linked Switchable Polymer for Fast and Highly Efficient Phosphorylated Peptide Enrichment

revised on 10.09.2020, 10:01 and posted on 10.09.2020, 10:09 by Li-Jun Chen, jun-long zhu, Fan-Fan Zhu, Xu-Qing Wang, Jin Wen, Hai-Bo Yang, Philip Gale

The selective and efficient capture of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis. However, this remains a significant challenge due to the inherently low abundance of these species in complex bio-samples. In this paper, we report a switchable two-dimensional (2D) supramolecular polymer that can serve as an ideal platform for the enrichment of phosphopeptides. A positively charged metallacycle incorporated into the polymer endows the material with a high affinity for phosphopeptides. Importantly, the stimuli-responsive nature of the polymer facilitates switchable binding affinity of phosphopeptides, resulting in its excellent performance in phosphopeptide enrichment and separation from model proteins. The polymer has a high enrichment capacity (165 mg/g) and detection sensitivity (2 fmol), high enrichment recovery (88%), excellent specificity, and rapid enrichment and separation properties. Additionally, we have demonstrated the capture of phosphopeptides from the tryptic digest of real bio-samples illustrating the potential of this polymeric material in phosphoproteomic studies.


Selective anion transporters with therapeutic potential

Australian Research Council

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Organic supramolecular and aggregate chemistry

National Natural Science Foundation of China

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Innovation Program of Shanghai Municipal Education Commission 2019-01-07-00-05-E00012

Austrian Science Fund (FWF) Project M2709-N28


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The University of Sydney



ORCID For Submitting Author


Declaration of Conflict of Interest

There are no conflicts of interest