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Displacement Aptamer Paper.pdf (1.02 MB)
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A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

preprint
submitted on 24.08.2020 and posted on 25.08.2020 by Rhushabh Maugi, bernadette gamble, david bunka, Mark Platt
A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

History

Email Address of Submitting Author

m.platt@lboro.ac.uk

Institution

loughborough University

Country

UK

ORCID For Submitting Author

0000-0002-9195-7216

Declaration of Conflict of Interest

the authors declare no conflict of interest

Version Notes

presubmission draft

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