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Furse et al. 2021 31P lipidomics pipeline_manuscript.docx (1.83 MB)

A Pipeline for Making 31P NMR Accessible for Small- and Large-Scale Lipidomics Studies

preprint
submitted on 18.02.2021, 10:46 and posted on 19.02.2021, 06:37 by Samuel Furse, Huw Williams, Adam Watkins, Samuel Virtue, Antonio Vidal-Puig, Marika Charalambous, Albert Koulman
Detailed molecular analysis is of increasing importance in research into the regulation of biochemical pathways, organismal growth and disease. Lipidomics in particular is increasingly sought after as it provides insight into molecular species involved in energy storage, signalling and fundamental cellular structures. This has led to the use of a range of tools and techniques to acquire lipidomics data. 31P NMR for lipidomics offers well-resolved head group/lipid class analysis, structural data that can be used to inform and strengthen interpretation of mass spectrometry data and part of structural determination a priori. In the present study, we codify the use of 31P NMR for lipidomics to make the technique more accessible to new users and more useful for a wider range of studies. We describe the process from sample extraction to data processing and analysis. This pipeline is important because it allows greater thoroughness in lipidomics studies and increases scope for answering scientific questions about lipid systems.

Funding

The influence of diet on the honeybee lipidome

Biotechnology and Biological Sciences Research Council

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The validation of biomarkers of metabolic efficacy in infant nutrition

Biotechnology and Biological Sciences Research Council

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RG_18_7_33636

Communication between mother and fetus : Imprinting and endocrine adaptations to pregnancy

Medical Research Council

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History

Email Address of Submitting Author

samuel@samuelfurse.com

Institution

RBG Kew, University of Cambridge

Country

United Kingdom

ORCID For Submitting Author

0000-0003-4267-2051

Declaration of Conflict of Interest

No conflicts

Version Notes

Pre-peer-reviewed final version

Exports

ChemRxiv

Exports