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A New Interpretation of the Absorption and the Dual Fluorescence of Prodan in Solution

preprint
submitted on 12.08.2020 and posted on 13.08.2020 by Cintia Vequi-Suplicy, Yoelvis Orozco-González, M. Teresa Lamy, Sylvio Canuto, Kaline Coutinho
Prodan is a fluorescent probe used to monitor biological systems such as lipid membrane,
proteins and DNA. Remarkable interest is associated to the interpretation of its fluorescent spectrum.
In this paper the sequential hybrid Quantum Mechanics/Molecular Mechanics (S-QM/MM) method was used to establish that the fluorescent emission occurs from two different excited states, resulting in a broad asymmetric emission spectrum with two transitions. The absorption spectra in several solvents were measured and calculated using different theoretical models and they all agree that the first observed band is composed of three electronic excitation very close energetically to each other.

Funding

FAPESP 2010/08365-0

FAPESP 2006/55513-9

FAPESP 2012/15161-7

FAPESP 2017/11631-2

FAPESP 2014/50983-3

CAPES 23038.004630/2014-35

CNPq 141260/2017-3

History

Email Address of Submitting Author

cintia.vequi@imdea.org

Institution

Universidade de São Paulo

Country

Brasil

ORCID For Submitting Author

0000-0003-1980-5039

Declaration of Conflict of Interest

The following article has been submitted to The Journal of Chemical Physics. After it is published, it will be found at https://aip.scitation.org/journal/jcp

Version Notes

The following article has been submitted to The Journal of Chemical Physics. After it is published, it will be found at https://aip.scitation.org/journal/jcp

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