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A graphenic and potentiometric sensor for monitoring the growth of bacterial biofilms.pdf (596.26 kB)
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A Graphenic and Potentiometric Sensor for Monitoring the Growth of Bacterial Biofilms

preprint
submitted on 26.04.2020 and posted on 27.04.2020 by Noemi Violeta Poma Sajama, Federico Maria Vivaldi, Andrea Bonini, Pietro Salvo, Bernardo Melai, Daria Bottai, Arianna Tavanti, Fabio Di Francesco

Biofilm development on surfaces represents one of the most challenging issues in the medical field. The irreversibility of this process, and the characteristics of the biofilm, make biofilm associated infections difficult to treat and prone to relapse. Thanks to the short response time, low- cost, easy fabrication and potential for miniaturization, electrochemical sensors are expected to play an important role in monitoring biofilm formation. Here, we describe the use of a potentiometric sensor based on functionalized reduced graphene oxide for monitoring the formation and growth of biofilms from three bacterial species (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) in liquid and semisolid culture media. The Open Circuit Potential (OCP) between an Ag/AgCl pseudo reference electrode and a working electrode, both screen-printed on a flexible polyethylene terephthalate film, was measured during the growth of bacteria. The OCP decreased over time as bacteria grew on the sensor surface exhibiting a negative correlation

Funding

Fondazione Pisa through the SEMPRE project.

History

Email Address of Submitting Author

federicomaria.vivaldi@phd.unipi.it

Institution

Universty of Pisa

Country

Italy

ORCID For Submitting Author

0000-0003-1304-9893

Declaration of Conflict of Interest

no conflict of interest

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