These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
A Different Protein Corona Cloaks “True-to-Life” Nanoplastics with Respect to Synthetic Polystyrene Nanobeads
preprintsubmitted on 26.04.2021, 20:47 and posted on 28.04.2021, 13:25 by Serena Ducoli, Stefania Federici, Roland Nicsanu, Andrea Zendrini, Claudio Marchesi, Lucia Paolini, Annalisa Radeghieri, Paolo Bergese, Laura Eleonora Depero
Given the complexity of separating nanoplastics from environmental samples, studies have usually been conducted using synthetic polystyrene nanobeads. By mechanical fragmentation in cryogenic conditions of daily-life plastic items, we produced “true-to-life” nanoplastics (T2LNPs), that promises to give a true insight into the interaction with biological systems. T2LNPs have been fully characterized by Fourier transform Infrared spectroscopy and by Atomic Force Microscopy. They result in populations of spheroidal nanoparticles with a broad multimodal size distribution. The mandatory need for a representative sample to evaluate the potential effects of nanoparticles on human health and the environment is demonstrated by the different protein corona identified on T2LNPs and synthetic polystyrene nanobeads upon incubation with human plasma.