These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
ChemRxiv.pdf (1.03 MB)

A Designed Protein Binding-Pocket to Control Photochemical Isomerization

submitted on 02.10.2018, 14:26 and posted on 03.10.2018, 13:08 by Bryan J. Lampkin, Cecilia Monteiro, Evan T. Powers, Paige M. Bouc, Jeffery W. Kelly, Brett VanVeller

ESIPT involves a photochemical isomerization and creates the opportunity for the emission of two distinct wavelengths of light from a single fluorophore. The selectivity between these two wavelengths of emission is dependent on the environment around the fluorophore and suggests the possibility for ratiometric monitoring of protein microenvironments. Unfortunately, nonspecific binding of ESIPT fluorophores does not often lead to dramatic changes in the ratio between the two wavelengths of emission. A protein binding pocket was designed to selectively discriminate between the two channels of emission available to an ESIPT fluorophore. More broadly, this work demonstrates that specific interactions between the protein and the fluorophore are essential to realize strong ratiometric differences between the two possible wavelengths of emission. The design strategies proposed here lead to an ESIPT fluorophore that can discern subtle differences in the interface between two proteins.


Email Address of Submitting Author


Iowa State University



ORCID For Submitting Author


Declaration of Conflict of Interest

ETP and JWK receive royalty payments from a patent for ta- famidis.