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ChemrXiv - ITGaVb5 Manuscript final.pdf (2.84 MB)

A CycloRGDf(Me-V) Analog as Chemical Probe to Study Integrins Function in Living Cells

submitted on 09.01.2020 and posted on 13.01.2020 by Vojtech Kapras, Wyatt T. Slattery, Frederic Menard

We report a chemical probe that can be used to image integrins in living cells. The fluorescent probe was derived from cyclo-RGDf(Me-V), a compound selective for integrins that possess an RGD-binding domain. We describe its synthesis and we demonstrate its use to detect integrin αVβ5 in cells. The probe’s dissociation constant for the integrin αVβ5 protein is 0.18 μM. The probe's activity was validated in murine BV-2 microglial cells using cell engulfment assays, flow cytometry, and confocal fluorescence imaging. This probe will provide access to spatiotemporally resolved studies of RGD-binding integrin function in living cells without the need for genetic modification.


Email Address of Submitting Author


University of British Columbia



ORCID For Submitting Author


Declaration of Conflict of Interest

No conflict of interest


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