A Blueprint for the Synthesis and Characterisation of Thin Graphene Oxide with Controlled Lateral Dimensions for Biomedicine

We report the reproducible production of graphene oxide flakes of controlled lateral dimensions under endotoxin free conditions for biomedical and hazard assessment investigations. In addition, we report the thorough characterisation of those materials, using optical, atomic force, electron microcopies; fluorescence, UV/Vis and Raman spectroscopies; Zeta potential, FTIR, TGA, and XPS. We identified that a combination of microscopic techniques are needed to fully assess the lateral dimensions of GO flakes.