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Small Molecule-Inducible RNA-Targeting Systems for Temporal Control of RNA Regulation in Vivo

preprint
submitted on 21.01.2020 and posted on 22.01.2020 by Simone Rauch, Krysten A. Jones, Bryan Dickinson
RNA regulation is critical for gene expression control, but tools to temporally manipulate RNA regulatory mechanisms are lacking. Here, we present small molecule-inducible RNA-targeting effectors based on our previously developed CRISPR/Cas-inspired RNA targeting system (CIRTS), which can trigger RNA editing, degradation, or translation on target transcripts. We go on to show the inducible CIRTS editor can be deployed for RNA base editing in vivo, providing a new set of tools to probe RNA regulatory dynamics and control gene expression.

Funding

R35GM119840

R01MH122142

History

Email Address of Submitting Author

dickinson@uchicago.edu

Institution

University of Chicago

Country

USA

ORCID For Submitting Author

0000-0002-9616-1911

Declaration of Conflict of Interest

B.C.D. and S.R. have filed a provisional patent on CIRTS.

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