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Rational Enzyme Design Without Structural Knowledge: A Sequence-Based Approach for Efficient Generation of Glycosylation Catalysts

preprint
revised on 24.03.2020 and posted on 24.03.2020 by David Teze, Zhao, Jiao, Mathias Wiemann, Zubaida Gulshan Kazi, Rossana Lupo, Mette Errebo Rønne, Göran Carlström, Jens Øllgaard Duus, Yves-Henri Sanejouand, Michael J. O’Donohue, Eva Nordberg Karlsson, Régis Fauré, Henrik Stålbrand, Birte Svensson

We present an enzyme engineering approach based solely on amino-acids sequence to convert glycoside hydrolases into transglycosylases. We demonstrate its effectiveness on enzymes form five different glycoside hydrolase families, synthesizing various oligosaccharides containing different α-/β-pyranosides or furanosides in one-step with high yields.

Funding

NNF17OC0025660

History

Email Address of Submitting Author

david.teze@gmail.com

Institution

Technical University of Denmark

Country

Denmark

ORCID For Submitting Author

0000-0002-6865-6108

Declaration of Conflict of Interest

The authors declare no conflict of interest.

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