Cambridge Open Engage home
What is Cambridge Open Engage?
How to Submit
Browse
About
News [opens in a new tab]

RNA Phosphorothioate Modification in Prokaryotes and Eukaryotes

14 January 2020, Version 2
Working Paper
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

We report the first RNA phosphorothioate modification in both prokaryotes and eukaryotes. The GpsG modification exists in the Rp configuration and was quantified with liquid chromatography coupled with tandem mass spectrometry. Furthermore, we show the Dnd clusters that regulate DNA PT modification also play roles in RNA PT modification.

Keywords

RNA
post-transcriptional modifications
Phosphorothioate

Supplementary materials

Title
Description
Actions
Title
PT Supplementary Information
Description
Actions
Title
Seq DndA knock out
Description
Actions

Comments

Comments are not moderated before they are posted, but they can be removed by the site moderators if they are found to be in contravention of our Commenting Policy [opens in a new tab] - please read this policy before you post. Comments should be used for scholarly discussion of the content in question. You can find more information about how to use the commenting feature here [opens in a new tab] .
This site is protected by reCAPTCHA and the Google Privacy Policy [opens in a new tab] and Terms of Service [opens in a new tab] apply.

Now Published

RNA Phosphorothioate Modification in Prokaryotes and Eukaryotes [opens in a new tab]
Ying Wu, Yaning Tang, Xiaolong Dong, Ya Ying Zheng, Phensinee Haruehanroengra, Song Mao, Qishan Lin, Jia Sheng journal article
ACS Chemical Biology , Volume 15, Issue 6
Online publication date: Apr 10, 2020

Version History

Jan 14, 2020 Version 2

Metrics

3,610
796
0
Views
Downloads
Citations

License

CC logo
CC
BY logo
BY
NC logo
NC
ND logo
ND
The content is available under CC BY NC ND 4.0 [opens in a new tab]

DOI

10.26434/chemrxiv.11574099.v2
D O I: 10.26434/chemrxiv.11574099.v2 [opens in a new tab]

Funding

NSF-1845486

Author’s competing interest statement

The authors declare no conflict of interest.

Share