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submitted on 07.01.2020 and posted on 08.01.2020by Ryosuke Ueki, Shota Hayashi, Masaya Tsunoda, Momoko Akiyama, Hanrui Liu, Tasuku Ueno, Yasuteru Urano, Shinsuke Sando
Optochemical tools that can modulate activity of the target protein
provide an opportunity for studying and regulating the related biological
processes. Here we present a DNA-based nongenetic optochemical tool that can
control dynamics of growth factor signaling. A photo-caged DNA aptamer for a
growth factor receptor was designed to assemble into an active dimer form,
depending on UV irradiation, thereby enabling optical control of the
dimerization and subsequent activation of the receptor. This photo-caged
mimicry of growth factor can be a promising tool for elucidating a linkage
between dynamics of signaling and resulting biological outcomes, as well as for
manipulating cellular functions and the fate of living cells.