ChemRxiv
These are preliminary reports that have not been peer-reviewed. They should not be regarded as conclusive, guide clinical practice/health-related behavior, or be reported in news media as established information. For more information, please see our FAQs.
Functionalization.pdf (925.34 kB)

Functionalization of Peptide-coated Platinum Nanoparticles for Various Biological Applications

preprint
revised on 07.08.2019 and posted on 07.08.2019 by Michal Shoshan, Mao Li, Thomas Vonderach, Helma Wennemers

Functionalizing noble metal nanoparticles is often necessary to tailor their properties, in particular for biological applications such as drug delivery, therapy and imaging. However, this process is highly challenging, due to the complications in maintaining similar geometrical parameters of the nanoparticles as of without functionalization. The heptapeptide H-Lys-[Pro-Gly-Lys]2-NH2 was found to be an efficient additive for the formation and stabilization of small and monodisperse platinum nanoparticles (PtNPs). We show that the same additive peptide can be used as a platform for the functionalization of PtNPs, as demonstrated by the introducion of three different moieties: coumarin for monitoring cellular uptake of the PtNPs; biotin for detecting their stability in human blood serum; and an arginine-containing peptide for enhanced cell penetration. The peptide-coated PtNPs can be functionalized both before and after reduction of the metal ions, in aqueus environment, emphasizing the high robustness of the system.


Funding

Marie Sklodowska-Curie grant agreement No 705970

History

Email Address of Submitting Author

michal.shoshan@gmail.com

Institution

ETH Zurich

Country

Switzerland

ORCID For Submitting Author

0000-0002-6944-3913

Declaration of Conflict of Interest

No conflict of interest

Exports

Logo branding

Exports