Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR

08 November 2019, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Phospholipid quantification by 31P NMR and lipid separation and detection by LC-MS/MS. Fatty acid quantification using scripted reconstruction of elution profile for each fatty acid associated with each lipid species. The combination of NMR and and MS data is complementary and allows better quantification and more complete description than each approach would allow by itself. Datasets exists for both whole-cell and plasma membrane fraction, and we also describe and document the extraction techniques.

Keywords

Lipidomics
neurolipidomics
SH-SY5Y
NMR spectroscopy methods
LC-MS/MS

Supplementary materials

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Supplementary MJ2 2019 01 16
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SH5 lipidomics submission revision
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Supplementary revision
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Supplementary weblinks

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