Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR

Phospholipid quantification by 31P NMR and lipid separation and detection by LC-MS/MS. Fatty acid quantification using scripted reconstruction of elution profile for each fatty acid associated with each lipid species. The combination of NMR and and MS data is complementary and allows better quantification and more complete description than each approach would allow by itself. Datasets exists for both whole-cell and plasma membrane fraction, and we also describe and document the extraction techniques.