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Enzymatic Formation of an Artificial Base Pair Using a Modified Adenine Nucleoside Triphosphate

preprint
submitted on 22.12.2019, 00:37 and posted on 23.12.2019, 22:33 by Marie Flamme, Pascal Röthlisberger, Fabienne Levi-Acobas, Mohit Chawla, Romina Oliva, Luigi Cavallo, Gilles Gasser, Philippe Marlière, Piet Herdewijn, Marcel Hollenstein
The expansion of the genetic alphabet with additional, unnatural base pairs (UBPs) is an important and long standing goal in synthetic biology. Nucleotides acting as ligands for the coordination of metal cations have advanced as promising candidates for such an expansion of the genetic alphabet. However,the inclusion of artificial metal base pairs in nucleic acids mainly relies on solid-phase synthesis approaches and very little is known on polymerase-mediated synthesis. Herein, we report on the selective and high yielding enzymatic construction of a silver-mediated base pair as well as a two-step protocol for the synthesis of DNA duplexes containing a metal UBP. Guided by DFT calculations, we also shed light into the mechanism of formation of this UBP as well as into the structural and energetic preferences. Even though this silver UBP is not directly amenable to in vitro selection experiments, the enzymatic synthesis of this UBP provides valuable insights for the design of future, more potent systems aiming at expanding the genetic alphabet.

Funding

Institut Pasteur Paris

doctoral school MTCI University of Paris Descartes

King Abdullah University of Science and Technology (KAUST)

MIUR-FFABR (Fondo per il Finanziamento Attività Base di Ricerca)

ERC Consolidator Grant PhotoMedMet (GA 681679)

ANR-10-IDEX-0001-02 PSL

History

Email Address of Submitting Author

marcel.hollenstein@pasteur.fr

Institution

Institut Pasteur

Country

France

ORCID For Submitting Author

0000-0003-0263-9206

Declaration of Conflict of Interest

The authors declare no competing interests

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