ESIPT-Based Fluorescence Probe for the Ratiometric Detection of Superoxide

A simple ESIPT-based fluorescence probe (HMBT-LW) was developed for the detection of superoxide (O2⸱-). HMBT-LW was synthesised over two steps and was shown to rapidly detect low concentrations of O2⸱- (limit of detection = 7.4 μM), fully reacting within two minutes. Furthermore, HMBT-LW demonstrated excellent selectivity and sensitivity towards O2⸱-.