Dynamics of Individual Molecular Shuttles Under Mechanical Force

Here, we exploit the high force (0.1 pN), spatial (1 nm) and temporal (1 kHz) resolutions of optical tweezers<a href="#_ENREF_4"><sup></sup></a><sup></sup><a href="#_ENREF_11"><sup></sup></a> to quantify and control mechanically the real-time kinetics of individual synthetic molecular shuttles operating at near-physiological conditions, for several hundreds of switching cycles, near equilibrium conditions.