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submitted on 06.08.2019 and posted on 06.08.2019by Nobutaka Fujieda, Sachiko Yanagisawa, Minoru Kubo, Genji Kurisu, Shinobu Itoh
To unveil the activation of dioxygen on the copper centre (Cu2O2core) of tyrosinase, we performed X-ray crystallograpy with active-form tyrosinase at near atomic resolution. This study provided a novel insight into the catalytic mechanism of the tyrosinase, including the rearrangement of copper-oxygen species as well as the intramolecular migration of copper ion induced by substrate-binding.