Chemical Synthesis of Shiga Toxin Subunit B Using a Next-Generation Traceless “Helping Hand” Solubilizing Tag

23 September 2019, Version 2
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

This manuscript describes the development and characterization of a second-generation "helping hand" solubilizing tag. This semipermanent tag aids solubility of peptides during purification and handling steps and is compatible with common native chemical ligation conditions. The utility of this tag is demonstrated by the synthesis of the Shiga toxin B subunit.

Keywords

Native Chemical Ligation
Peptide synthesis
solubilizing groups
traceless tag
helping hand
Shiga toxin (Stx)
chemical Protein Synthesis

Supplementary materials

Title
Description
Actions
Title
HH2-Shiga ESI FINAL
Description
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HH2-main figures FINAL
Description
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