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Biological Relevance of Charge Transfer Branching Pathways in Photolyases

preprint
submitted on 01.03.2019 and posted on 04.03.2019 by Daniel Holub, Tilman Lamparter, Marcus Elstner, Natacha Gillet
In PhrA, a class III CPD photolyase, two branching tryptophan charge transfer pathways have been characterized in the mechanism of FAD photoreduction. To provide a molecular explanation of the charge transfer abilities of both pathways, we performed simulations where the protein motion and the positive charge are simultaneously propagated. Our computational approach reveals that one pathway drives a very fast charge transfer whereas the other pathway provides a very good thermodynamic stabilization of the positive charge. During the simulations, the positive charge firstly moves on the fast triad, allowing the stabilization of reduced FAD. After one nanosecond, we observe a nearly equal probability to find the charge at ending tryptophan of either pathway. Our results highlight the role of the protein environment, which manages the association of a kinetic and a thermodynamic pathways to trigger a fast and efficient FAD photoreduction.

History

Email Address of Submitting Author

natacha.gillet@kit.edu

Institution

Karlsruhe Institute of Technology

Country

Germany

ORCID For Submitting Author

0000-0002-7657-6861

Declaration of Conflict of Interest

No conflict of interest

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in Physical Chemistry Chemical Physics

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