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An Unprecedented Octahedral Trifluoromagnesate MgF3(Wat)– Transition State Analog Reveals The Molecular Mechanism of ATP Hydrolysis by Zika Virus Helicase

preprint
submitted on 27.02.2020 and posted on 28.02.2020 by Mengyu Ge, Robert W. Molt Jr, Huw T. Jenkins, G. Michael Blackburn, Yi Jin, Alfred A. Antson
Metal fluoride complexes mimic the transferring phosphoryl group in many enzyme-catalyzed reactions. We here employ the trifluoromagnesate transition state analog (TSA) to study a Zika virus NS3h helicase, which uses energy from ATP hydrolysis to reorganize ssRNA leading to completion of virus replication. The crystal structure of this TSA complex displays two conformations for a catalytically important loop, demonstrating how ATP hydrolysis can be coupled with RNA translocation. Unexpectedly, the trifluoromagnesate core of this transition state complex is octahedral. It is identified as having an unprecedented MgF3(Wat) ligand, confirmed by 19F NMR analysis. This structure was further probed by quantum mechanical calculations of the catalytic core (200 atoms), confirming the structural data interpretation and the concerted mechanism of ATP hydrolysis by this class of helicase. The formation of this MgF3(Wat) ligand in helicase but not in other multiple MFx structures for ATPases and GTPases strongly implies they cannot possess such an additional water in their active sites.

Funding

Wellcome Trust

Chinese Scholarship Council

History

Email Address of Submitting Author

jiny6@cardiff.ac.uk

Institution

Cardiff University

Country

United Kingdom

ORCID For Submitting Author

0000-0002-6927-4371

Declaration of Conflict of Interest

No conflict of Interest

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