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An Integrated Microfluidic Probe for Mass Spectrometry Imaging of Biological Samples

preprint
submitted on 08.05.2020 and posted on 13.05.2020 by Xiangtang Li, ruichuan yin, hang hu, Yingju Li, Xiaofei Sun, Sudhansu Dey, Julia Laskin
Ambient ionization based on liquid extraction is widely used in mass spectrometry
imaging (MSI) of molecules in biological samples. The development of nanospray desorption electrospray ionization (nano-DESI) has enabled the robust imaging of tissue sections with high spatial resolution. However, the fabrication of the nano-DESI probe is challenging, which limits its dissemination to the broader scientific community. Herein, we describe the design and performance of an integrated microfluidic probe (iMFP) for nano-DESI MSI. The glass iMFP fabricated using photolithography, wet etching, and polishing shows comparable performance to the capillary-based nano-DESI MSI in terms of stability and sensitivity; the spatial resolution of better than 25 μm was obtained in these first proof-of-principle experiments. The iMFP is easy to operate and align in front of a mass spectrometer, which will facilitate broader use of liquid extraction-based MSI in biological research, drug discovery, and clinical studies.

Funding

Novel Platform for Quantitative Subcellular Resolution Imaging of Human Tissues Using Mass Spectrometry

National Heart Lung and Blood Institute

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Molecular signaling in uterine receptivity to implantation

National Institute of Child Health and Human Development

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Endocannabinoid Signaling during Early Pregnancy

National Institute on Drug Abuse

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History

Email Address of Submitting Author

jlaskin@purdue.edu

Institution

Purdue University

Country

United States

ORCID For Submitting Author

0000-0002-4533-9644

Declaration of Conflict of Interest

No conflict of interest

Version Notes

revised version

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in Angewandte Chemie International Edition

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