A Dual Sensor Probe for the Sequential Detection of Copper(II) and Lactic Acid

18 April 2019, Version 1
This content is a preprint and has not undergone peer review at the time of posting.

Abstract

Herein, we report a novel fluorescent probe for the sequential detection of Copper II (Cu2+) and lactic acid. The probe based on acyl-thiourea derivative, N-((6-methoxypyridin-2-yl)carbamothioyl)benzamide (1) was synthesized, and its application as dual sensor for Cu2+ and lactic acid is reported. The dual detection by the probe is possible since 1 formed complex with Cu2+ and yielded yellow solution which disappeared after the addition of lactic acid. The probe exhibits yellow fluorescence with Cu2+ and LOD of Cu2+ with fluorescence is as low as 0.1ppm. The fluorescence is quenched after the addition of lactic acid and LOD of fluorescence quenching by lactate is as low as 1 ppm. Further, structural modification in the probe 1 suggest crucial role of both pyridine and acyl-thiourea moiety in the binding of Cu2+. Interestingly, 1 also assembles to fluorescent fibers which show tunable emission properties. These fibers are broken in the presence of Cu2+ and regenerated in the presence of lactic acid. Hence, disaggregation at molecular level might play a crucial role in causing yellow fluorescence in the presence of Cu2+. To, the best of our knowledge, this is the first report wherein a dual sensor for Cu2+ and lactate ion is synthesized and it may in all possibilities pave the way for diagnosis of Cu2+ associated disorders like Wilson’s disease and in the detection of elevated lactate levels which are associated with wide range of pathologies like mitochondrial diseases, cerebral ischemia and cancer.

Keywords

Copper detection
Lactic acid detection
Acyl thiourea
Fluorescence
Disaggregation

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