Eilertsen, Justin
Stroberg, Wylie
Schnell, Santiago
Phase-Plane Geometries in Coupled Enzyme Assays
The determination of a substrate or enzyme activity by coupling of one enzymatic reaction with another easily detectable (indicator) reaction is a common practice in the biochemical sciences. The dynamical behavior of couple enzyme catalyzed assays is studied by analysis in the phase plane. Usually, the kinetics of enzyme reactions is simplified with singular perturbation analysis to derive rate or time course expressions valid under the quasi-steady-state and reactant stationary state assumptions. In this paper, we analyze two types of time-dependent slow manifolds that occur in asymptotically autonomous vector fields that arise from enzyme coupled assays. We show that the motion of the slow manifolds relative to the motion of the solution must be taken into account in order to formulate accurate leading order asymptotic solutions. We also develop a rigorous mathematical framework from which to analyze enzyme catalyzed indicator reaction from couple enzyme assays.
Enzyme kinetics;Coupled enzyme assays;Michaelis-Menten reaction;Time-dependent slow manifold;differential-algebraic equations;asymptotically autonomous vector field;Chemistry;Biological Sciences;Mathematics
2018-02-26
https://chemrxiv.org/articles/Phase-Plane_Geometries_in_Coupled_Enzyme_Assays/5923786

10.26434/chemrxiv.5923786.v1