Optimal Duration of the Preincubation Phase in Enzyme Inhibition Experiments
KuzmicPetr
2020
This report describes an algebraic formula to
calculate the optimal duration of the pre-incubation phase in enzyme-inhibition
experiments, based on the assumed range of expected values for the dissociation
equilibrium constant of the enzymeâ€“inhibitor complex and for the bimolecular
association rate constant. Three typical experimental scenarios are treated, namely,
(1) single-point primary screening at relatively high inhibitor concentrations;
(2) dose-response secondary screening of relatively weakly bound inhibitors; (3)
dose-response screening of tightly-bound inhibitors.